Assays and Services Portfolio: The Singh Laboratory provides integrated, high-dimensional immune profiling and mechanistic assay platforms spanning cellular phenotyping, metabolism, spatial biology, and extracellular vesicle biology, enabling comprehensive dissection of immune regulation in translational and clinical contexts.
- High-Dimensional Immune Profiling (CyTOF & Spectral Flow): Comprehensive phenotyping of immune populations including regulatory T cells, Tr1 cells, and exhausted CD4/CD8 T cells using >35–40 parameter panels. Customized panel design and deep immunophenotyping across all species (human, NHPs and mouse) samples in whole blood, PBMCs, Tissues, clinical specimen with advanced analysis support.
2. Antigen-Specific T Cell Tracking (Tetramer-Based Assays): Quantification and characterization of donor- or antigen-specific T cell responses using MHC tetramers. Enables precise tracking of alloreactive or disease-relevant T cell populations in transplantation and immunotherapy studies.
3. Immune Metabolism & Bioenergetics Profiling (SCENITH/scMEP & Seahorse XF): Integrated single-cell and bulk metabolic analysis to assess glycolysis, oxidative phosphorylation, and fatty acid oxidation in immune cells. Combines SCENITH/scMEP with real-time Seahorse XF measurements (OCR/ECAR) to define metabolic fitness, activation states, and exhaustion-linked rewiring in T cells and myeloid populations.
4. Extracellular Vesicle (EV) Isolation & Characterization and Functional Assays: Quantification of cytokines, chemokines, and growth factors using platforms such as Olink and Luminex. Enables discovery of circulating immune signatures and translational biomarkers.
5. Immune Secretome & Biomarker Profiling (Multiplex Cytokine Assays): High-resolution mapping of immune cell localization and interactions within tissues using platforms such as Imaging Mass Cytometry (IMC), COMET/Lunapore, CODEX, and MIBI. Ideal for studying tissue-specific immune niches (e.g., Liver/ Graft tissue, Lymph nodes, clinical tissue biopsy, wound or tumor tissue).
6. Spatial Immune Profiling (Multiplex Imaging & Spatial Proteomics): High-resolution mapping of immune cell localization and interactions within tissues using platforms such as Imaging Mass Cytometry (IMC), COMET/Lunapore, CODEX, and MIBI. Ideal for studying tissue-specific immune niches (e.g., Liver/ Graft tissue, Lymph nodes, clinical tissue biopsy, wound or tumor tissue).
7. Preclinical, clinical Immune Modeling & Functional T Cell Assays (Human & NHPs Systems): Design and execution of immune studies in clinically relevant human samples and non-human primate models, integrated with functional assays including MLR, proliferation (CellTrace/CFSE), and intracellular cytokine profiling (IFN-γ, TNF-α, IL-17). Enables robust evaluation of immune regulation, tolerance induction, and effector dysfunction/exhaustion.
8. Computational Immunology, Study Design & Translational Consulting: Integrated support spanning advanced computational analysis and experimental strategy development, including single-cell trajectory mapping, ligand–receptor modeling (CellChat/NicheNet), and immune network inference. Provides end-to-end guidance on study design, immune monitoring, and panel development, with a strong focus on transplantation, immune tolerance, and immunometabolism.
